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Histological Changes Resulting From Infection With The Entamoeba Histolytica In Mice And The Effect Of Treatment With Extracts Of The Aloe Barbadensis Plant

Vol. 3 No. 2 (2025): December:
DOI : https://doi.org/10.21070/anamnetic.v3i2.1631
Published : Dec 5, 2025

Shahad Saad Daham (1)

(1) , Iraq
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Abstract:

The current study was designed to investigate the effect of aqueous and alcoholic extracts of Aloe barbadensis on the tissues of 4-8-week-old male Balb/c mice weighing 22-28 grams, collected from the beginning of September 2025 until October 2025. These mice were taken from the animal house of the College of Veterinary Medicine, Tikrit University, and placed in special cages in the animal house, which were bedded with wood shavings. Care was taken to keep the cages clean. Microscopic examination of tissue sections taken from mice infected with the Entamoeba histolytica parasite revealed pathological histological changes in the large intestine (colon) and liver. Specifically, necrosis of the intestinal mucosa was observed in the lamina propria, along with degeneration of several cells lining the intestinal glands. Large numbers of white blood cells were also found infiltrating the lamina propria between the glands, compared to the control group. The intestinal glands appeared tubular in shape with cavities containing mucous droplets. Between the bases and walls of the glands, mucous membranes were also found. In the liver of the positive (infected) control group, the portal vein appeared longitudinally wide and contained a mass of red blood cells and some white blood cells, with thickening of its wall adjacent to the bile duct, compared to the negative (uninfected) control group. Microscopic examination of histological sections taken from the intestines of mice infected and treated with an aqueous extract of Aloe barbadensis showed that the intestinal mucosa contained epithelial cells lining the intestinal lumen, with a large number of mucosal goblet cells distributed among the epithelial cells. In the infected liver treated with the aqueous extract, the liver cells appeared in long rows, each row containing normal-shaped hepatocytes with a polygonal appearance and a large, spherical nucleus. In the intestines of mice infected and treated with an alcoholic extract of Aloe barbadensis the intestinal mucosa showed numerous mucosal goblet cells and mucosal droplets in the cytoplasm of these cells, in addition to columnar epithelial cells facing the lumen. In the intestines and in the infected liver treated with the alcoholic extract, the liver tissue contained hepatocytes arranged in interlocking rows similar to honeycomb cells with large spherical nuclei, indicating the efficiency of the extracts in repairing damaged tissues.

References

1. Othman, N.; Ujang, J.A.; Ng, Y.l.; Kumarasamy, G. and Noordin, R.(2020). Amoebiasis. In Molecular Advancements in Tropical Diseases Drug Discovery (pp.1-19). Academic press.

2. Razavi,S. M.;tan, Arab, Z.N.; Khayatan, D.; Niknejad, A.H; Hosseini, Y.; Zare, K. and Sahebkar, A.(2024). An introduction to the pathophysiology of parasitic infection. In Advances in Antiparasitic Therapies and Drug Delivery (pp 1-32) Academic press.

3. Charles, H.; Prochazka, M. and Thorley,K.(2022). Outbreak of sexually transmitted, extensively drug-resistant Shigella Sonnei in the UK, 2021-22: a descriptive epidemiological study. Lancet Infect Dis: 22:1503-10.

4. Wesa, J.; Shuman, J.; Bastuzel, I.; Dickerson, J. and Ingram- Smith, C.(2021). Encystation of Entamoeba histolytica in Axenix Culture Microorganisms, 9(4),873.

5. Taherian, M.; Samankan, S. and Cagir, B.(2019). Amebic colitis. State Peals. Treasure Island: Stat Pearls Publishing. StatePearls Publishing LLC.

6. Ulah,R.; Shafiq, M.; Rehman, M.U.; Khan, I,; Hayat, A.; and Jehangir, I.(2022). The public Health and Clinical Importance of Amoebiasis. Health Science Journal, 16(3), 1-7

7. Matushkina, N.; Kuzmina, T.; Trokhymets, V,; and Khoperia, V.(2023). Basic information and Tutorial Exercises for Medical Parasitology Cambridge Scholars Publishing.

8. Singh K, Verma R, Gupta S, and Sharma A. (2023). Potential of aloe vera as a growth promoter in poultry: mechanistic insights. *Poult Sci J *. 55(1):47–55.

9. Salem R, El-Ashry M, Omara A. (2022). Aloe vera as a growth promoter and immune modulator in poultry. *Poultr Sci Int*. 3(1):56–68.

10. Khan, R.U.; Naz, S, De Marzo D.; Dimuccio, M.M.; Bozzo, G.; Tufarelli, V.; Losacco, C.; Ragni, M. (2023). Aloe vera: a sustainable green alternative to exclude antibiotics in modern poultry production. Antibiotics . 12(1):44.

11. Clark, C.G and Diamond,L.S (2002). Method for cultivation of huminal parasitic protists of clinical importance. Clinic .Microbial. Rev.15(2),329-341.

12. عقيل, محسن (2003). معجم الأعشاب المصور, الطبعة الاولى. مؤسسة الأعلمي للمطبوعات/ لبنان 305 ص

13. Ilbas, A. I. ; Gonen , U. ; Yilmaz, S. and Dadandi, M. T. Y.(2012). Cytotoxicity of Aloe vera gel extracts on Allium cepa root tip cells.J. Turkish.36(3): 263-268.

14. 14-الحاج, حميد احمد (2010). التحضيرات المجهرية الضوئية (التقانات المجهرية) الأسس النظرية والتطبيقات. الطبعة الأولى. مركز الكتب, عمان-الأردن.

15. Bancroft, J.D. and Stevens, A.(1982). Theory and practice of histological techniques,2 nd ed. Churchill Livingstone, London. pp 109-120.

16. Al-Hilfi, A.; Abu- Jameer, A.; Khalii, M.; Al-Malak, A and Abd-ulrahman, M.(2019). Histopathological study of invasive and non –invasive Entamoeba SPP. In experimental rats, Bulleitin of the National Research Center, 43(1),p.66.

17. Jorge-Rosas, F.; Diaz- Godines, C.; Garcia –Aguirre, S.; Martinez-Calvillo, S and Carrero, J.C.(2024). Entamoeba histolytica-induced NETs are highly cytotoxic on hepatic and colonic cells due to serine proteases and myeloperoxidase activities. Frontiers in Immunology, 15: 149-346.

18. Tavares, P.; Rigothier, M.; Khun, H. Roux, P.;Huerre, M. and Guillen, N.(2005). Roles of cell adhesion and cytoskeleton activity in Entamoeba histolytica pathogenesis: a delicate balance. Infect. Immun.73:1771-1778.

19. Khaleel, Z.I.; Abdul Wahhab, I.G.; Al-Bairuty, G.A.(2024). The effect of infection with Entamoeba histolytica on the level of some biological variables and histological changes in the liver. Journa of Biotecnology Research center ,Vol.18,No.2, pp.94-102.

20. Jahangeer,M.; Mahmood, Z.; Munir, N.; Waraich, U.E.A.; Tahir, I,M.; Akram M. and Zainab, R.(2020). Naegleria fowleri: Sources of infection Pathophysiology, diagnosis and management/; a review. Clinical and Experimental Pharmacology and Physiology, 47(2). 199-212.

21. - الشمري, عبد الله يونس معيوف,(2024). تأثير مستخلصات بذور اليقطين والمشمش ودقائق السلينيوم النان على طفيل الاميبا الحالة للنسيج داخل الجسم الحي. اطروحة دكتوراه, كلية التربية للعلوم الصرفة, جامعة تكريت.

22. 22- الداوودي، فرحان خليل حسين (2019). انتشار داء الزحار الاميبي بين الأطفال في مدينة كركوك مع محاولة علاجية بالمستخلص المائي لنباتي السعد والزعتر البري على أنسجة الفئران المختبرية المصابة. رسالة ماجستير. كلية التربية للبنات. جامعة تكريت.

23. Manvitha , K. and Bidya , B. (2014) . Aloe vera : a wonder its history , cultivation and medicinal uses J. Pharmacognosy and Phytochemistry , Vol. 2, No. 5, P.85,87.